Translational_Unit

Part:BBa_K5367001:Design

Designed by: Xinjie Shen   Group: iGEM24_NJU-China   (2024-09-28)


The TPS1 gene encodes Trehalose-6-phosphate synthase 1, an enzyme involved in the biosynthetic pathw


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Overexpressing TPS1 can enhance the stress resistance of Saccharomyces cerevisiae. Therefore, when designing primers to obtain this fragment from S288C, we focused solely on the DNA sequence from the start codon to the stop codon, omitting the preceding sequences. This allows us to freely add a strong promoter for improved overexpression.


Source

Natural fragments of Saccharomyces cerevisiae S288C.

References